Notice: This article uses content directly taken from the Antagonists in Season One article at the Vampire Diaries Wiki, and is licensed under the Creative Commons Attribution-Share Alike License.
| Character | Episodes | Appearances as Antagonist |
|---|---|---|
| Damon Salvatore | Pilot, The Night of the Comet, Friday Night Bites, Family Ties, You're Undead to Me, Lost Girls, Haunted, 162 Candles, History Repeating | (9/22) |
| Katherine Pierce | Lost Girls, Children of the Damned, Founder's Day | (3/4) |
| Vicki Donovan | Haunted | (1/7) |
| Logan Fell | Lost Girls, The Turning Point | (2/5) |
| Noah | Unpleasantville | (1/3) |
| Anna | Fool Me Once | (1/11) |
| Isobel Flemming | A Few Good Men, Isobel | (2/4) |
| Frederick | There Goes the Neighborhood, Let The Right One In | (2/2) |
| Bethanne | There Goes the Neighborhood | (1/2) |
| Tomb Vampires | Let The Right One In, Founder's Day | (1/2) |
| John Gilbert | Under Control, Blood Brothers, Isobel, Founder's Day | (4/5) |
| Stefan Salvatore | Miss Mystic Falls | (1/22) |
| Richard Lockwood | Founder's Day | (1/8) |